Wound~Seq

Wounds are often infected with multiple microorganisms that complicate and prolong the healing process. Granger Genetics is proud to launch Wound~Seq, a molecular diagnostic test using up to date reference databases for identification of bacteria/ fungi and determination of antibiotic resistance genes. This Next Generation Care test increases the ability to diagnose pathogenic agents affecting wound healing. Traditional bacterial identification uses culturing techniques that have been used for nearly 100 years. Although widely utilized, physicians realize that culturing often fails to identify infections. This is because the majority (85%-95%) of bacteria cannot grow in culture and therefore cannot be identified by traditional culture methods.

Wound~Seq does not rely on bacterial culture but rather uses state of the art DNA sequencing technology to identify all bacteria present in the wound. Scientists at Granger Genetics developed a proprietary approach for sequencing organisms infecting a wound and identification of the infecting microorganisms. This assay is capable of detecting offending organisms across a wide range of concentrations. The test is commercially available and provides actionable results in 48 hours (typically) of sample receipt.

Wound~Seq Requisition Form
Wound~Seq Flyer 1     Wound~Seq Flyer 2     Wound~Seq Flyer 3

What is Wound~Seq?


Identifying and managing infections is an important aspect affecting all healthcare professionals that treat patients with a wound. The diagnosis of infection is often made clinically and laboratory testing is used to identify offending organisms that cause the infection. It is important to remember that the skin is the largest organ in the body and an important function is to prevent bacteria from entering into our system. A wound can become contaminated and infected through direct contact with a contaminated surface such as our clothes and hands. These bacteria can replicate and overwhelm our immune systems and invade the surrounding tissue.

All chronic wounds are contaminated with bacteria and are subject to infection. Infected wounds are often critically colonized with multiple microorganisms including bacteria and fungi. Traditionally, physicians have relied on wound cultures to identify organisms that cause infections. However, we now know that culturing fails to identify 85-95% of all bacteria. Physicians relying on culture reports to treat patients are not seeing the entire picture. Molecular diagnostics does not rely on the ability of a bacteria to grow in culture and therefore can accurately identify organisms in a wound.

How Does It Work?


Scientists at Granger Genetics developed a proprietary approach for sequencing organisms infecting a wound and identification of the infecting microorganisms. This assay is capable of detecting offending organisms across a wide range of concentrations. Wound~Seq does not rely on bacterial culture but rather utilizes state of the art DNA sequencing technology.

In addition to determining presence or absence of organisms, Wound~Seq identifies key antibacterial resistance genes that may be present in the wound that can affect the success of treatment. Wound~Seq can easily be implemented into your practice without the need to purchase expensive equipment or additional supplies. Granger Genetics provides the collection kits to your office/medical center and covers the cost of shipment to the laboratory. Results are available through our proprietary Edox system and can easily be accessed with any web browser.

ICD10 Codes

  • S91 Open wound of ankle, foot and toes
    • S91.0 Open wound of ankle
    • S91.1 Open wound of toe without damage to nail
    • S91.2 Open wound of toe with damage to nail
    • S91.3 Open wound of foot
  • M01.X7: Direct infection of tarsus, metatarsus and phalanges in infectious and parasitic diseases classified elsewhere
  • L00-L08 Infections of the skin and subcutaneous tissue

Chronic Osteomyelitis

  • M86.3 Chronic multifocal osteomyelitis
  • M86.4 Chronic osteomyelitis with draining sinus
  • M86.5 Other chronic hematogenous osteomyelitis
  • M86.6 Other chronic osteomyelitis

Non-pressure chronic ulcer of unspecified heel and midfoot limited to breakdown of skin

  • L97.402 Non-pressure chronic ulcer of unspecified heel and midfoot with fat layer exposed
  • L97.403 Non-pressure chronic ulcer of unspecified heel and midfoot with necrosis of muscle
  • L97.404 Non-pressure chronic ulcer of unspecified heel and midfoot with necrosis of bone
  • L97.409 Non-pressure chronic ulcer of unspecified heel and midfoot with unspecified severity
  • L97.411 Non-pressure chronic ulcer of right heel and midfoot limited to breakdown of skin
  • L97.412 Non-pressure chronic ulcer of right heel and midfoot with fat layer exposed
  • L97.413 Non-pressure chronic ulcer of right heel and midfoot with necrosis of muscle
  • L97.414 Non-pressure chronic ulcer of right heel and midfoot with necrosis of bone
  • L97.419 Non-pressure chronic ulcer of right heel and midfoot with unspecified severity
  • L97.421 Non-pressure chronic ulcer of left heel and midfoot limited to breakdown of skin
  • L97.422 Non-pressure chronic ulcer of left heel and midfoot with fat layer exposed
  • L97.423 Non-pressure chronic ulcer of left heel and midfoot with necrosis of muscle
  • L97.424 Non-pressure chronic ulcer of left heel and midfoot with necrosis of bone
  • L97.429 Non-pressure chronic ulcer of left heel and midfoot with unspecified severity